alr [2019-07-23 09:46:40]

alanine racemase

Locus: BSU_04640

Isoelectric point: 8.43

Molecular weight: 43.10 kDa

Protein length: 389 aa

Gene length: 1167 bp

Function: peptidoglycan biosynthesis

Product: alanine racemase

Essential: yes

E.C.: 5.1.1.1

Synonyms: dal,alrA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan
• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/acquisition of L- and D-alanine
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan
• Groups of genes → Essential genes

Gene

Coordinates: 517,372 → 518,541

Phenotypes of a mutant

• reported to be essential PubMed, however deletion mutants have been reported by Ferrari et al. (1985) and Xia et al (2007) PubMed
• auxotrophy for D-alanine

The protein

Catalyzed reaction/ biological activity

• L-alanine = D-alanine (according to Swiss-Prot)

Protein family

• alanine racemase family (with YncD, according to UniProt)

Paralogous protein(s)

• YncD

Cofactors

• PLP (according to UniProt)

Structure

• 1L6G (with N-[5�-phosphopyridoxyl]-D-alanine, Geobacillus stearothermophilus), 1SFT (Geobacillus stearothermophilus)

Expression and Regulation

Operons

• Genes: alr-ndoAI-ndoA
  Description: PubMed

Biological materials

Mutant

• alr-1, two-base mutation 526AG>T (=R176X), creating a frameshift and an immediate stop codon, available in BGSC 1A4, a D-alanine auxotroph

References

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